Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice

Tiia Koho; Minni R L Koivunen; Sami Oikarinen; Laura Kummola; Selina Mäkinen; Anssi J. Mähönen; Amirbabak Sioofy-Khojine; Varpu Marjomäki; Artur Kazmertsuk; Ilkka Junttila; Markku S. Kulomaa; Heikki Hyöty; Vesa P. Hytönen; Olli H. Laitinen

doi:10.1016/j.antiviral.2014.01.013

Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice

Tiia Koho
, Minni R L Koivunen
, Sami Oikarinen
, Laura Kummola
, Selina Mäkinen
, Anssi J. Mähönen
, Amirbabak Sioofy-Khojine
, Varpu Marjomäki
, Artur Kazmertsuk
, Ilkka Junttila
, Markku S. Kulomaa
, Heikki Hyöty
, Vesa P. Hytönen
, Olli H. Laitinen

Tutkimustuotos: Artikkeli › Tieteellinen › vertaisarvioitu

40 Sitaatiot (Scopus)

Abstrakti

Coxsackievirus B3 (CVB3) is an important cause of acute and chronic viral myocarditis, and dilated cardiomyopathy (DCM). Although vaccination against CVB3 could significantly reduce the incidence of serious or fatal viral myocarditis and various other diseases associated with CVB3 infection, there is currently no vaccine or therapeutic reagent in clinical use. In this study, we contributed towards the development of a CVB3 vaccine by establishing an efficient and scalable ion exchange chromatography-based purification method for CVB3 virus and baculovirus-insect cell-expressed CVB3 virus-like particles (VLPs). This purification system is especially relevant for vaccine development and production on an industrial scale. The produced VLPs were characterized using a number of biophysical methods and exhibited excellent quality and high purity. Immunization of mice with VLPs elicited a strong immune response, demonstrating the excellent vaccine potential of these VLPs.

Alkuperäiskieli	Englanti
Sivut	93-101
Sivumäärä	9
Julkaisu	Antiviral Research
Vuosikerta	104
Numero	1
DOI - pysyväislinkit	https://doi.org/10.1016/j.antiviral.2014.01.013
Tila	Julkaistu - 2014
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

YK:n kestävän kehityksen tavoitteet

Tämä tuotos edistää seuraavia kestävän kehityksen tavoitteita:

SDG 3 – Hyvä terveys ja hyvinvointi

Tutkimusalat

CVB3
Ion exchange chromatography
VLP

Julkaisufoorumi-taso

Jufo-taso 1

Pääsy asiakirjaan

10.1016/j.antiviral.2014.01.013

Siteeraa tätä

Koho, T., Koivunen, M. R. L., Oikarinen, S., Kummola, L., Mäkinen, S., Mähönen, A. J., Sioofy-Khojine, A., Marjomäki, V., Kazmertsuk, A., Junttila, I., Kulomaa, M. S., Hyöty, H., Hytönen, V. P., & Laitinen, O. H. (2014). Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice. Antiviral Research, 104(1), 93-101. https://doi.org/10.1016/j.antiviral.2014.01.013

@article{b591ca79ac3548f0b87fad91f132c160,

title = "Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice",

abstract = "Coxsackievirus B3 (CVB3) is an important cause of acute and chronic viral myocarditis, and dilated cardiomyopathy (DCM). Although vaccination against CVB3 could significantly reduce the incidence of serious or fatal viral myocarditis and various other diseases associated with CVB3 infection, there is currently no vaccine or therapeutic reagent in clinical use. In this study, we contributed towards the development of a CVB3 vaccine by establishing an efficient and scalable ion exchange chromatography-based purification method for CVB3 virus and baculovirus-insect cell-expressed CVB3 virus-like particles (VLPs). This purification system is especially relevant for vaccine development and production on an industrial scale. The produced VLPs were characterized using a number of biophysical methods and exhibited excellent quality and high purity. Immunization of mice with VLPs elicited a strong immune response, demonstrating the excellent vaccine potential of these VLPs.",

keywords = "CVB3, Ion exchange chromatography, VLP, CVB3, Ion exchange chromatography, VLP",

author = "Tiia Koho and Koivunen, \{Minni R L\} and Sami Oikarinen and Laura Kummola and Selina M{\"a}kinen and M{\"a}h{\"o}nen, \{Anssi J.\} and Amirbabak Sioofy-Khojine and Varpu Marjom{\"a}ki and Artur Kazmertsuk and Ilkka Junttila and Kulomaa, \{Markku S.\} and Heikki Hy{\"o}ty and Hyt{\"o}nen, \{Vesa P.\} and Laitinen, \{Olli H.\}",

year = "2014",

doi = "10.1016/j.antiviral.2014.01.013",

language = "English",

volume = "104",

pages = "93--101",

journal = "Antiviral Research",

issn = "0166-3542",

publisher = "Elsevier B.V.",

number = "1",

}

Koho, T, Koivunen, MRL, Oikarinen, S , Kummola, L, Mäkinen, S, Mähönen, AJ, Sioofy-Khojine, A, Marjomäki, V, Kazmertsuk, A, Junttila, I, Kulomaa, MS, Hyöty, H , Hytönen, VP & Laitinen, OH 2014, 'Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice', Antiviral Research, Vuosikerta 104, Nro 1, Sivut 93-101. https://doi.org/10.1016/j.antiviral.2014.01.013

TY - JOUR

T1 - Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice

AU - Koho, Tiia

AU - Koivunen, Minni R L

AU - Oikarinen, Sami

AU - Kummola, Laura

AU - Mäkinen, Selina

AU - Mähönen, Anssi J.

AU - Sioofy-Khojine, Amirbabak

AU - Marjomäki, Varpu

AU - Kazmertsuk, Artur

AU - Junttila, Ilkka

AU - Kulomaa, Markku S.

AU - Hyöty, Heikki

AU - Hytönen, Vesa P.

AU - Laitinen, Olli H.

PY - 2014

Y1 - 2014

N2 - Coxsackievirus B3 (CVB3) is an important cause of acute and chronic viral myocarditis, and dilated cardiomyopathy (DCM). Although vaccination against CVB3 could significantly reduce the incidence of serious or fatal viral myocarditis and various other diseases associated with CVB3 infection, there is currently no vaccine or therapeutic reagent in clinical use. In this study, we contributed towards the development of a CVB3 vaccine by establishing an efficient and scalable ion exchange chromatography-based purification method for CVB3 virus and baculovirus-insect cell-expressed CVB3 virus-like particles (VLPs). This purification system is especially relevant for vaccine development and production on an industrial scale. The produced VLPs were characterized using a number of biophysical methods and exhibited excellent quality and high purity. Immunization of mice with VLPs elicited a strong immune response, demonstrating the excellent vaccine potential of these VLPs.

AB - Coxsackievirus B3 (CVB3) is an important cause of acute and chronic viral myocarditis, and dilated cardiomyopathy (DCM). Although vaccination against CVB3 could significantly reduce the incidence of serious or fatal viral myocarditis and various other diseases associated with CVB3 infection, there is currently no vaccine or therapeutic reagent in clinical use. In this study, we contributed towards the development of a CVB3 vaccine by establishing an efficient and scalable ion exchange chromatography-based purification method for CVB3 virus and baculovirus-insect cell-expressed CVB3 virus-like particles (VLPs). This purification system is especially relevant for vaccine development and production on an industrial scale. The produced VLPs were characterized using a number of biophysical methods and exhibited excellent quality and high purity. Immunization of mice with VLPs elicited a strong immune response, demonstrating the excellent vaccine potential of these VLPs.

KW - CVB3

KW - Ion exchange chromatography

KW - VLP

KW - CVB3

KW - Ion exchange chromatography

KW - VLP

U2 - 10.1016/j.antiviral.2014.01.013

DO - 10.1016/j.antiviral.2014.01.013

M3 - Article

AN - SCOPUS:84894116625

SN - 0166-3542

VL - 104

SP - 93

EP - 101

JO - Antiviral Research

JF - Antiviral Research

IS - 1

ER -

Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice

Abstrakti

YK:n kestävän kehityksen tavoitteet

Tutkimusalat

Julkaisufoorumi-taso

Pääsy asiakirjaan

Sormenjälki

Siteeraa tätä