Effect of Previous Alkylating Agent Exposure on Follicle Numbers in Cryopreserved Prepubertal and Young Adult Ovarian Tissue after Long-Term Xenografting

Mirja Nurmio, Babak Asadi-Azarbaijani, Mi Hou, Ronja Kiviö, Jorma Toppari, Helena Tinkanen, Tiina Laine, Irma C. Oskam, Kirsi Jahnukainen

    Tutkimustuotos: ArtikkeliTieteellinenvertaisarvioitu

    4 Sitaatiot (Scopus)
    5 Lataukset (Pure)

    Abstrakti

    Purpose and methods: To elucidate whether previous cancer treatment affects graft recovery and follicle numbers, morphology, and development in grafts, cryopreserved ovarian biopsies obtained from 18 cancer patients aged 1–24 years with and without exposure to chemotherapy were xenografted as 1 mm3 fragments to immunodeficient mice for 22 weeks with exogenous stimulation. Results: Graft recovery showed no association with chemotherapy exposure, pubertal stage, or leukemia contamination. Total follicle number per recovered graft varied between 0 and 1031 in the chemotherapy-exposed and between 0 and 502 in the non-chemotherapy-exposed group. Atretic follicles formed the largest proportion of the follicle pool in chemotherapy-exposed grafts. Increased atresia correlated with exposure to alkylating agents (mean ± SD 8866.2 ± 9316.3 mg/m2) but not with anthracyclines, pubertal stage, or leukemia contamination. Conclusion: The observation confirms the harmful effects of alkylating agents on ovarian tissue. Therapy at the median cumulative dose of 8866 mg/m2 leads to the decreased quality of cryopreserved ovarian follicles in children and young adults.

    AlkuperäiskieliEnglanti
    Artikkeli399
    Sivumäärä13
    JulkaisuCancers
    Vuosikerta14
    Numero2
    DOI - pysyväislinkit
    TilaJulkaistu - tammik. 2022
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

    Julkaisufoorumi-taso

    • Jufo-taso 1

    !!ASJC Scopus subject areas

    • Oncology
    • Cancer Research

    Sormenjälki

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