TY - JOUR
T1 - Electrophysiological evaluation of human induced pluripotent stem cell-derived cardiomyocytes obtained by different methods
AU - Prajapati, Chandra
AU - Ojala, Marisa
AU - Lappi, Henna
AU - Aalto-Setälä, Katriina
AU - Pekkanen-Mattila, Mari
N1 - Funding Information:
This study was funded by the Finnish Foundation for Cardiovascular Research, the Finnish Funding Agency for Technology and Innovation, Competitive Research Funding of the Pirkanmaa Hospital District, Biocenter Finland, Finnish Cultural Foundation and Aarne Koskelo Foundation. Biocenter Finland Stem cell core is also acknowledged. We would like to acknowledge Markus Haponen, Merja Lehtinen and Annika Länsivaara for the technical support.
Publisher Copyright:
© 2021 The Authors
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/3
Y1 - 2021/3
N2 - The human induced pluripotent stem cells (hiPSCs) derived cardiomyocytes (CMs) (hiPSC-CMs) retain the same genetic information as the donor, and they have been shown to faithfully recapitulate the disease phenotypes of various genetic cardiac diseases. The hiPSC-CMs can be utilized in multiple types of studies and in most cases, the functionality of hiPSC-CMs is of interest. For the functional analyses, the hiPSC-CMs need to be manipulated after differentiation, e.g. enriched or dissociated into single-cell stage. For the functional assessments to be reliable and reproducible, the cell culture environment should support the cells in an optimal manner. The aim of the present study was to evaluate the effect of various differentiation methods, as well as coating materials used for the dissociated cells on the functionality of the differentiated hiPSC-CMs. The different protocols not only had different differentiation efficiencies, but they also yielded functionally different hiPSC-CMs. Additionally, the coating material had a major effect on the functionality of the hiPSC-CMs. The results of the present study emphasize that the cardiac differentiation method and the coating material have a major effect on hiPS-CMs’ characteristics. Thus, when different hiPSC lines and results obtained in different labs are compared, extra care should be taken to check the conditions when results are compared.
AB - The human induced pluripotent stem cells (hiPSCs) derived cardiomyocytes (CMs) (hiPSC-CMs) retain the same genetic information as the donor, and they have been shown to faithfully recapitulate the disease phenotypes of various genetic cardiac diseases. The hiPSC-CMs can be utilized in multiple types of studies and in most cases, the functionality of hiPSC-CMs is of interest. For the functional analyses, the hiPSC-CMs need to be manipulated after differentiation, e.g. enriched or dissociated into single-cell stage. For the functional assessments to be reliable and reproducible, the cell culture environment should support the cells in an optimal manner. The aim of the present study was to evaluate the effect of various differentiation methods, as well as coating materials used for the dissociated cells on the functionality of the differentiated hiPSC-CMs. The different protocols not only had different differentiation efficiencies, but they also yielded functionally different hiPSC-CMs. Additionally, the coating material had a major effect on the functionality of the hiPSC-CMs. The results of the present study emphasize that the cardiac differentiation method and the coating material have a major effect on hiPS-CMs’ characteristics. Thus, when different hiPSC lines and results obtained in different labs are compared, extra care should be taken to check the conditions when results are compared.
KW - Cardiomyocyte
KW - Differentiation
KW - Electrophysiology
KW - Human induced pluripotent stem cells
U2 - 10.1016/j.scr.2021.102176
DO - 10.1016/j.scr.2021.102176
M3 - Article
AN - SCOPUS:85099607869
SN - 1873-5061
VL - 51
JO - Stem Cell Research
JF - Stem Cell Research
M1 - 102176
ER -
