Fabrication and developmentof emulsion-templated porous materials for 3D cell culture

Tutkimustuotos: AbstraktiScientific


Microphysiological systems (MPS) have been developed tostudy the primary functions of human organs in vitro. This study aims toevaluate the application of emulsion-templated porous biomaterials in MPS. Porous biomaterials are among the most well-known and attractive materials forstudying in vitro models. A suitable 3D bio-scaffold is known to be used fortissue regeneration, providing a 3D substrate for better cell attachment,nutrition flow, infiltration, and cell migration within the host tissue [1]. 3Dstructures with pore sizes in the range of 100-500 µm (macropores) provide goodinterconnectivity to allow cell attachment on the surface, tissue ingrowth, andvascularization [2]. It was also aimed to fabricate and develop highly porousbiomaterials in macro sizes from high internal phase emulsions (HIPEs)consisting of more than 75% of the volume; hence, such materials have greatdemand in the field of MPS and regenerative medicine. A combination ofmacroporous emulsion-templated biomaterials with MPS technology has thepotential to facilitate mass and oxygen transfer into the tissues and enable anovel 3D platform for various cell types and vascularization compared tobiomaterials with pore sizes < 100 µm. Thus, there is a demand for versatilematerial with flexible porous structures, mechanical, and chemicalcharacteristics. The current state of the project showed that the fabricatedbiomaterial indicates good morphology and characteristics, making it feasiblefor its application in MPS. The rheological properties of the material understudy were evaluated, and the fabricated scaffolds were characterized bymicro-CT and SEM. The observed pore size was around 250-350 µm, supporting theapplication of this material as a 3D environment for in vitro studies.

TilaJulkaistu - 2024
OKM-julkaisutyyppiEi OKM-tyyppiä
TapahtumaMicrophysiological Systems World Summit - Berlin, Saksa
Kesto: 26 kesäk. 202330 kesäk. 2023
Konferenssinumero: 2023


ConferenceMicrophysiological Systems World Summit
LyhennettäMPS World Summit


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