Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) hold great potential in the cardiovascular field for human disease modeling, drug development, and regenerative medicine. However, multiple hurdles still exist for the effective utilization of hiPSC-CMs as a human-based experimental platform that can be an alternative to the current animal models. To further expand their potential as a research tool and bridge the translational gap, we have generated a cardiac-specific hiPSC reporter line that differentiates into fluorescent CMs using CRISPR-Cas9 genome editing technology. The CMs illuminated with the mScarlet fluorescence enable their non-invasive continuous tracking and functional cellular phenotyping, offering a real-time 2D/3D imaging platform. Utilizing the reporter CMs, we developed an imaging-based cardiotoxicity screening system that can monitor distinct drug-induced structural toxicity and CM viability in real time. The reporter fluorescence enabled visualization of sarcomeric disarray and displayed a drug dose-dependent decrease in its fluorescence. The study also has demonstrated the reporter CMs as a biomaterial cytocompatibility analysis tool that can monitor dynamic cell behavior and maturity of hiPSC-CMs cultured in various biomaterial scaffolds. This versatile cardiac imaging tool that enables real time tracking and high-resolution imaging of CMs has significant potential in disease modeling, drug screening, and toxicology testing.
- Jufo-taso 1
!!ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
SormenjälkiSukella tutkimusaiheisiin 'Fluorescent hiPSC-derived MYH6-mScarlet cardiomyocytes for real-time tracking, imaging, and cardiotoxicity assays'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.
- 1 Konferenssiesitelmä
Janne T. Koivisto (Speaker), Reeja Maria Cherian (Contributor), Martta Häkli (Contributor), Jenny Parraga Meneses (Contributor), Jennika Karvinen (Contributor), Christine Gering (Contributor), Chandra Prajapati (Contributor), Risto-Pekka Pölönen (Contributor), Birhanu Belay (Contributor), Jari Hyttinen (Contributor), Mari Pekkanen-Mattila (Contributor), Minna Kellomäki (Contributor) & Katriina Aalto-Setälä (Contributor)22 maalisk. 2023