Human induced pluripotent stem cell-derived cardiomyocytes as an electrophysiological model: Opportunities and challenges—The Hamburg perspective

Djemail Ismaili; Carl Schulz; András Horváth; Jussi T. Koivumäki; Delphine Mika; Arne Hansen; Thomas Eschenhagen; Torsten Christ

doi:10.3389/fphys.2023.1132165

Human induced pluripotent stem cell-derived cardiomyocytes as an electrophysiological model: Opportunities and challenges—The Hamburg perspective

Djemail Ismaili
, Carl Schulz
, András Horváth
, Jussi T. Koivumäki
, Delphine Mika
, Arne Hansen
, Thomas Eschenhagen
, Torsten Christ^*

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Katsausartikkeli › vertaisarvioitu

13 Sitaatiot (Scopus)

36 Lataukset (Pure)

Abstrakti

Models based on human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are proposed in almost any field of physiology and pharmacology. The development of human induced pluripotent stem cell-derived cardiomyocytes is expected to become a step forward to increase the translational power of cardiovascular research. Importantly they should allow to study genetic effects on an electrophysiological background close to the human situation. However, biological and methodological issues revealed when human induced pluripotent stem cell-derived cardiomyocytes were used in experimental electrophysiology. We will discuss some of the challenges that should be considered when human induced pluripotent stem cell-derived cardiomyocytes will be used as a physiological model.

Alkuperäiskieli	Englanti
Artikkeli	1132165
Sivumäärä	10
Julkaisu	Frontiers in Physiology
Vuosikerta	14
DOI - pysyväislinkit	https://doi.org/10.3389/fphys.2023.1132165
Tila	Julkaistu - helmik. 2023
OKM-julkaisutyyppi	A2 Katsausartikkeli tieteellisessä aikakauslehdessä

Rahoitus

This study was supported by the German Ministry of Education and Research (BMBF, FKZ161L0236), by the German Centre for Cardiovascular Research (Grant No. 81Z0710110 to CS), European Research Council (ERC-AG IndivuHeart), Deutsche Forschungsgemeinschaft (DFG Es 88/12-1, DFG HA 3423/5-1), the Freie und Hansestadt Hamburg and the Fédération Française de Cardiologie (to DM).

Julkaisufoorumi-taso

Jufo-taso 1

!!ASJC Scopus subject areas

Physiology
Physiology (medical)

Pääsy asiakirjaan

10.3389/fphys.2023.1132165Lisenssi: CC BY

fphys-14-1132165Final published version, 837 KBLisenssi: CC BY

https://urn.fi/URN:NBN:fi:tuni-202303283248Lisenssi: CC BY

Siteeraa tätä

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title = "Human induced pluripotent stem cell-derived cardiomyocytes as an electrophysiological model: Opportunities and challenges—The Hamburg perspective",

abstract = "Models based on human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are proposed in almost any field of physiology and pharmacology. The development of human induced pluripotent stem cell-derived cardiomyocytes is expected to become a step forward to increase the translational power of cardiovascular research. Importantly they should allow to study genetic effects on an electrophysiological background close to the human situation. However, biological and methodological issues revealed when human induced pluripotent stem cell-derived cardiomyocytes were used in experimental electrophysiology. We will discuss some of the challenges that should be considered when human induced pluripotent stem cell-derived cardiomyocytes will be used as a physiological model.",

keywords = "action potentials, automated patch clamp, computer modelling and simulation, hESC-CM, hiPSC-CM, human cardiomyocytes, ion currents",

author = "Djemail Ismaili and Carl Schulz and Andr{\'a}s Horv{\'a}th and Koivum{\"a}ki, \{Jussi T.\} and Delphine Mika and Arne Hansen and Thomas Eschenhagen and Torsten Christ",

note = "Funding Information: This study was supported by the German Ministry of Education and Research (BMBF, FKZ161L0236), by the German Centre for Cardiovascular Research (Grant No. 81Z0710110 to CS), European Research Council (ERC-AG IndivuHeart), Deutsche Forschungsgemeinschaft (DFG Es 88/12-1, DFG HA 3423/5-1), the Freie und Hansestadt Hamburg and the F{\'e}d{\'e}ration Fran{\c c}aise de Cardiologie (to DM). Publisher Copyright: Copyright {\textcopyright} 2023 Ismaili, Schulz, Horv{\'a}th, Koivum{\"a}ki, Mika, Hansen, Eschenhagen and Christ.",

year = "2023",

month = feb,

doi = "10.3389/fphys.2023.1132165",

language = "English",

volume = "14",

journal = "Frontiers in Physiology",

issn = "1664-042X",

publisher = "Frontiers Media S.A.",

}

TY - JOUR

T1 - Human induced pluripotent stem cell-derived cardiomyocytes as an electrophysiological model

T2 - Opportunities and challenges—The Hamburg perspective

AU - Ismaili, Djemail

AU - Schulz, Carl

AU - Horváth, András

AU - Koivumäki, Jussi T.

AU - Mika, Delphine

AU - Hansen, Arne

AU - Eschenhagen, Thomas

AU - Christ, Torsten

N1 - Funding Information: This study was supported by the German Ministry of Education and Research (BMBF, FKZ161L0236), by the German Centre for Cardiovascular Research (Grant No. 81Z0710110 to CS), European Research Council (ERC-AG IndivuHeart), Deutsche Forschungsgemeinschaft (DFG Es 88/12-1, DFG HA 3423/5-1), the Freie und Hansestadt Hamburg and the Fédération Française de Cardiologie (to DM). Publisher Copyright: Copyright © 2023 Ismaili, Schulz, Horváth, Koivumäki, Mika, Hansen, Eschenhagen and Christ.

PY - 2023/2

Y1 - 2023/2

N2 - Models based on human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are proposed in almost any field of physiology and pharmacology. The development of human induced pluripotent stem cell-derived cardiomyocytes is expected to become a step forward to increase the translational power of cardiovascular research. Importantly they should allow to study genetic effects on an electrophysiological background close to the human situation. However, biological and methodological issues revealed when human induced pluripotent stem cell-derived cardiomyocytes were used in experimental electrophysiology. We will discuss some of the challenges that should be considered when human induced pluripotent stem cell-derived cardiomyocytes will be used as a physiological model.

AB - Models based on human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are proposed in almost any field of physiology and pharmacology. The development of human induced pluripotent stem cell-derived cardiomyocytes is expected to become a step forward to increase the translational power of cardiovascular research. Importantly they should allow to study genetic effects on an electrophysiological background close to the human situation. However, biological and methodological issues revealed when human induced pluripotent stem cell-derived cardiomyocytes were used in experimental electrophysiology. We will discuss some of the challenges that should be considered when human induced pluripotent stem cell-derived cardiomyocytes will be used as a physiological model.

KW - action potentials

KW - automated patch clamp

KW - computer modelling and simulation

KW - hESC-CM

KW - hiPSC-CM

KW - human cardiomyocytes

KW - ion currents

U2 - 10.3389/fphys.2023.1132165

DO - 10.3389/fphys.2023.1132165

M3 - Review Article

AN - SCOPUS:85149487738

SN - 1664-042X

VL - 14

JO - Frontiers in Physiology

JF - Frontiers in Physiology

M1 - 1132165

ER -

Human induced pluripotent stem cell-derived cardiomyocytes as an electrophysiological model: Opportunities and challenges—The Hamburg perspective

Abstrakti

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!!ASJC Scopus subject areas

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