@inbook{ae7e4235d35744a69a25ec12e5f488bf,
title = "Human intestinal organoid models for celiac disease research",
abstract = "Celiac disease pathogenesis, in addition to immune cell component, encompasses pathogenic events also in the duodenal epithelium. In celiac disease patients, exposure to dietary gluten induces drastic changes in epithelial differentiation and elicit cellular response to inflammatory cytokines. The autoantigen in celiac disease, transglutaminase 2 (TG2) enzyme, has been also suggested to play its pathogenic gliadin deamidation event in the intestinal epithelium. Therefore in vitro epithelial cell-line models have been exploited in the past to study these pathogenic mechanisms, but they are hampered by their simplistic nature lacking proper cell-type composition and intestinal environ. Moreover, these cell models harbor many cancer-related mutations in tumor suppressor genes making them unsuitable for studying cell differentiation. Intestinal organoids provide a near-native epithelial cell model to study pathogenic agents and mechanisms related to celiac disease. Here we describe protocols to initiate and maintain celiac patient-derived organoid cultures and how to grow them in alternative ways allowing their exploitation in different kind of experiments.",
keywords = "Celiac disease, Enterovirus infection, Epithelial differentiation, Intestinal organoids",
author = "Valeriia Dotsenko and Sioofy-Khojine, {Amir Babak} and Heikki Hy{\"o}ty and Keijo Viiri",
note = "Funding Information: This work was supported by the Academy of Finland (no. 310011, 337582), Competitive State Research Financing of the Expert Responsibility Area of Tampere University Hospital (Grant No. 9AC098). This project has received funding from the European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement No: 874864.The funding sources played no role in the design or execution of this study or in the analysis and interpretation of the data. Publisher Copyright: {\textcopyright} 2023 Elsevier Inc.",
year = "2023",
doi = "10.1016/bs.mcb.2023.01.008",
language = "English",
isbn = "978-0-443-19202-9",
series = "Methods in Cell Biology",
publisher = "Academic Press",
pages = "173--193",
editor = "Ainara Castellanos-Rubio and Lorenzo Galluzzi",
booktitle = "Celiac Disease",
}