Human intestinal organoid models for celiac disease research

Tutkimustuotos: LukuTieteellinenvertaisarvioitu

6 Sitaatiot (Scopus)

Abstrakti

Celiac disease pathogenesis, in addition to immune cell component, encompasses pathogenic events also in the duodenal epithelium. In celiac disease patients, exposure to dietary gluten induces drastic changes in epithelial differentiation and elicit cellular response to inflammatory cytokines. The autoantigen in celiac disease, transglutaminase 2 (TG2) enzyme, has been also suggested to play its pathogenic gliadin deamidation event in the intestinal epithelium. Therefore in vitro epithelial cell-line models have been exploited in the past to study these pathogenic mechanisms, but they are hampered by their simplistic nature lacking proper cell-type composition and intestinal environ. Moreover, these cell models harbor many cancer-related mutations in tumor suppressor genes making them unsuitable for studying cell differentiation. Intestinal organoids provide a near-native epithelial cell model to study pathogenic agents and mechanisms related to celiac disease. Here we describe protocols to initiate and maintain celiac patient-derived organoid cultures and how to grow them in alternative ways allowing their exploitation in different kind of experiments.

AlkuperäiskieliEnglanti
OtsikkoCeliac Disease
ToimittajatAinara Castellanos-Rubio, Lorenzo Galluzzi
KustantajaAcademic Press
Luku14
Sivut173-193
Sivumäärä21
ISBN (painettu)978-0-443-19202-9
DOI - pysyväislinkit
TilaJulkaistu - 2023
OKM-julkaisutyyppiA3 Kirjan tai muun kokoomateoksen osa

Julkaisusarja

NimiMethods in Cell Biology
Vuosikerta179
ISSN (painettu)0091-679X

Julkaisufoorumi-taso

  • Jufo-taso 1

!!ASJC Scopus subject areas

  • Cell Biology

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