In vitro platelet activation, aggregation and platelet-granulocyte complex formation induced by surface modified single-walled carbon nanotubes

Janos Fent, Peter Bihari, Minnamari Vippola, Essi Sarlin, Susan Lakatos

    Tutkimustuotos: ArtikkeliTieteellinenvertaisarvioitu

    8 Sitaatiot (Scopus)

    Abstrakti

    Surface modification of single-walled carbon nanotubes (SWCNTs) such as carboxylation, amidation, hydroxylation and pegylation is used to reduce the nanotube toxicity and render them more suitable for biomedical applications than their pristine counterparts. Toxicity can be manifested in platelet activation as it has been shown for SWCNTs. However, the effect of various surface modifications on the platelet activating potential of SWCNTs has not been tested yet. In vitro platelet activation (CD62P) as well as the platelet-granulocyte complex formation (CD15/CD41 double positivity) in human whole blood were measured by flow cytometry in the presence of 0.1 mg/ml of pristine or various surface modified SWCNTs. The effect of various SWCNTs was tested by whole blood impedance aggregometry, too. All tested SWCNTs but the hydroxylated ones activate platelets and promote platelet-granulocyte complex formation in vitro. Carboxylated, pegylated and pristine SWCNTs induce whole blood aggregation as well. Although pegylation is preferred from biomedical point of view, among the samples tested by us pegylated SWCNTs induced far the most prominent activation and a well detectable aggregation of platelets in whole blood. (C) 2015 Elsevier Ltd. All rights reserved.

    AlkuperäiskieliEnglanti
    Sivut1132-1139
    Sivumäärä8
    JulkaisuToxicology in Vitro
    Vuosikerta29
    Numero5
    DOI - pysyväislinkit
    TilaJulkaistu - elok. 2015
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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