Internalization and antigen presentation by mouse dendritic cells of rotavirus VP6 preparations differing in nanostructure

Kirsi Tamminen, Suvi Heinimäki, Stina Gröhn, Vesna Blazevic

    Tutkimustuotos: ArtikkeliScientificvertaisarvioitu

    3 Sitaatiot (Scopus)

    Abstrakti

    Nanoparticles are highly immunogenic due to the multivalent, repetitive antigen expression and direct activation of antigen presenting cells (APCs), key players of adaptive immune responses. Different virus-like particles (VLPs) have been used as display platforms to amplify immune responses to biologically relevant, but poorly immunogenic foreign antigens. A candidate platform based on rotavirus (RV) inner-capsid protein VP6 oligomers, such as nanotubes (T-VP6) and nanospheres (S-VP6), is also considered. Different VP6 nanostructures were compared for internalization and antigen presentation by the APCs. We found, that a lack of a high-order structures, T-VP6 and S-VP6, did not negatively affect VP6 uptake and presentation by murine bone-marrow derived dendritic cells (BMDCs) in vitro. Furthermore, T-VP6 was preferable to norovirus (NoV) VLPs for BMDC internalization resulting in significantly higher VP6-specific immune responses when T-VP6 and NoV VLP pulsed BMDCs were transferred to syngeneic mice. These results support the use of different VP6 nanostructures as foreign antigen delivery platforms.

    AlkuperäiskieliEnglanti
    Sivut26-31
    Sivumäärä6
    JulkaisuMOLECULAR IMMUNOLOGY
    Vuosikerta123
    DOI - pysyväislinkit
    TilaJulkaistu - heinäk. 2020
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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