TY - JOUR
T1 - Langmuir-Schaefer film deposition onto honeycomb porous films for retinal tissue engineering
AU - Ilmarinen, Tanja
AU - Vuorimaa-Laukkanen, Elina
AU - Talvitie, Elina
AU - Hakola, Hanna M.
AU - Skottman, Heli
AU - Kellomäki, Minna
PY - 2017
Y1 - 2017
N2 - Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye – the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch’s membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability. Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE. Throughout the study, microporous films consistently showed better results as cell culture materials for hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch’s membranes for hESC-RPE transplantation.
AB - Age-related macular degeneration (AMD) is the leading cause of vision loss in senior citizens in the developed world. The disease is characterised by the degeneration of a specific cell layer at the back of the eye – the retinal pigment epithelium (RPE), which is essential in retinal function. The most promising therapeutic option to restore the lost vision is considered to be RPE cell transplantation. This work focuses on the development of biodegradable biomaterials with similar properties to the native Bruch’s membrane as carriers for RPE cells. In particular, the breath figure (BF) method was used to create semi-permeable microporous films, which were thereafter used as the substrate for the consecutive Langmuir-Schaefer (LS) deposition of highly organised layers of collagen type I and collagen type IV. The newly developed biomaterials were further characterised in terms of surface porosity, roughness, hydrophilicity, collagen distribution, diffusion properties and hydrolytic stability. Human embryonic stem cell-derived RPE cells (hESC-RPE) cultured on the biomaterials showed good adhesion, spreading and morphology, as well as the expression of specific protein markers. Cell function was additionally confirmed by the assessment of the phagocytic capacity of hESC-RPE. Throughout the study, microporous films consistently showed better results as cell culture materials for hESC-RPE than dip-coated controls. This work demonstrates the potential of the BF-LS combined technologies to create biomimetic prosthetic Bruch’s membranes for hESC-RPE transplantation.
KW - Biomaterials
KW - Films
KW - Honeycomb films
KW - Langmuir-Schaefer film
KW - Langmuir-Schaefer films
KW - Pluripotent stem cells
KW - Retinal pigment epithelial cell
KW - Retinal pigment epithelium
KW - Tissue Engineering
KW - Tissue engineering
KW - Biomaterials
KW - Films
KW - Honeycomb films
KW - Langmuir-Schaefer film
KW - Langmuir-Schaefer films
KW - Pluripotent stem cells
KW - Retinal pigment epithelial cell
KW - Retinal pigment epithelium
KW - Tissue Engineering
KW - Tissue engineering
U2 - 10.1016/j.actbio.2017.02.035
DO - 10.1016/j.actbio.2017.02.035
M3 - Article
VL - 54
SP - 138
EP - 149
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
ER -