Methods for in vitro functional analysis of iPSC derived cardiomyocytes — Special focus on analyzing the mechanical beating behavior

    Tutkimustuotos: ArtikkeliScientificvertaisarvioitu

    28 Sitaatiot (Scopus)
    112 Lataukset (Pure)

    Abstrakti

    Abstract A rapidly increasing number of papers describing novel iPSC models for cardiac diseases are being published. To be able to understand the disease mechanisms in more detail, we should also take the full advantage of the various methods for analyzing these cell models. The traditionally and commonly used electrophysiological analysis methods have been recently accompanied by novel approaches for analyzing the mechanical beatingbehavior of the cardiomyocytes. In this review, we provide first a concise overview on the methodology for cardiomyocyte functional analysis and then concentrate on the video microscopy, which provides a promise for a new faster yet reliable method for cardiomyocyte functional analysis. We also show how analysis conditions may affect the results. Development of the methodology not only serves the basic research on the disease models, but could also provide the much needed efficient early phase screening method for cardiac safety toxicology. This article is part of a Special Issue entitled: Cardiomyocyte Biology: Integration of Developmental and Environmental Cues in the Heart edited by Marcus Schaub and Hughes Abriel.
    AlkuperäiskieliEnglanti
    Sivut1864-72
    JulkaisuBiochimica et Biophysica Acta: Molecular Cell Research
    Vuosikerta1863
    Numero7 Pt B
    DOI - pysyväislinkit
    TilaJulkaistu - 2015
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

    Tutkimusalat

    • Disease modeling
    • Induced pluripotent stem cells
    • Safety toxicology
    • Stem cell derived cardiomyocytes
    • Video microscopy

    Julkaisufoorumi-taso

    • Jufo-taso 1

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