Microflow-Based Device for In Vitro and Ex Vivo Drug Permeability Studies

Samu Hemmilä, Marika Ruponen, Elisa Toropainen, Unni Tengvall-Unadike, Arto Urtti, Pasi Kallio

Tutkimustuotos: ArticleScientificvertaisarvioitu

1 Sitaatiot (Scopus)

Abstrakti

This paper presents a novel microflow-based concept for studying the permeability of in vitro cell models or ex vivo tissues. Using the proposed concept, we demonstrate how to maintain physiologically relevant test conditions and produce highly reproducible permeability values for a range (31) of drug compounds. The apparent permeability coefficients (Papp) showed excellent correlation (0.89) with the values from experiments performed with a conventional Ussing chamber. Additionally, the microflow-based concept produces notably more concentrated samples than the conventional Ussing chamber-based approach, despite the fact that more than 10 times smaller quantities of test compounds and biological membranes are needed in the microflow-based concept.

AlkuperäiskieliEnglanti
Sivut455–462
Sivumäärä8
JulkaisuSLAS Technology
Vuosikerta25
Numero5
DOI - pysyväislinkit
TilaJulkaistu - 2020
OKM-julkaisutyyppiA1 Alkuperäisartikkeli

Julkaisufoorumi-taso

  • Jufo-taso 1

!!ASJC Scopus subject areas

  • Computer Science Applications
  • Medical Laboratory Technology

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