Mitochondrial temperature homeostasis resists external metabolic stresses

Mügen Terzioglu, Kristo Veeroja, Toni Montonen, Teemu O. Ihalainen, Tiina S. Salminen, Paule Bénit, Pierre Rustin, Young Tae Chang, Takeharu Nagai, Howard T. Jacobs

    Tutkimustuotos: ArtikkeliScientificvertaisarvioitu

    3 Sitaatiot (Scopus)
    12 Lataukset (Pure)

    Abstrakti

    Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric fluorescent temperature indicator targeted to mitochondria, the temperature of active mitochondria in four mammalian and one insect cell line was estimated to be up to 15°C above that of the external environment to which the cells were exposed. High mitochondrial temperature was maintained in the face of a variety of metabolic stresses, including substrate starvation or modification, decreased ATP demand due to inhibition of cytosolic protein synthesis, inhibition of the mitochondrial adenine nucleotide transporter and, if an auxiliary pathway for electron transfer was available via the alternative oxidase, even respiratory poisons acting down-stream of oxidative phosphorylation (OXPHOS) complex I. We propose that the high temperature of active mitochondria is an inescapable consequence of the biochemistry of OXPHOS and is homeo-statically maintained as a primary feature of mitochondrial metabolism.

    AlkuperäiskieliEnglanti
    ArtikkeliRP89232
    JulkaisueLIFE
    Vuosikerta12
    DOI - pysyväislinkit
    TilaJulkaistu - 2023
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

    Julkaisufoorumi-taso

    • Jufo-taso 2

    !!ASJC Scopus subject areas

    • Yleinen neurotiede
    • Yleinen biokemia, genetiikka ja molekyylibiologia
    • Yleinen immunologia ja mikrobiologia

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