Multiresolution MAPEM method for 3D reconstruction of symmetrical particles with electron microscopy

E. Acar, M. A. Baikoghli, M. Stark, S. Peltonen, U. Ruotsalainen, R. H. Cheng

    Tutkimustuotos: KonferenssiartikkeliScientificvertaisarvioitu

    Abstrakti

    The resolution and accuracy of the 3D images obtained with single particle reconstruction (SPR) highly depend on the number and signal to noise ratio of the particle images. The maximum a posteriori probability expectation maximization (MAPEM) reconstruction methods have been successful in suppressing noise and compensating for the limited angular sampling. This paper presents a multiresolution MAPEM (mMAPEM) method to improve the resolution and accuracy of the 3D images of the symmetrical particles reconstructed using SPR. The method utilizes the median root prior and the symmetry information about the reconstructed structure in the image domain. The method was compared with the conventional Fourier Reconstruction (FR) method using phantom and experimental datasets for different noise levels and projection angle sampling conditions. The numerical and visual assessment of the reconstruction results demonstrate that the mMAPEM method provides more accurate results than FR.

    AlkuperäiskieliEnglanti
    OtsikkoEMBEC and NBC 2017 - Joint Conference of the European Medical and Biological Engineering Conference EMBEC 2017 and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics, NBC 2017
    KustantajaSpringer Verlag
    Sivut141-144
    Sivumäärä4
    ISBN (painettu)9789811051210
    DOI - pysyväislinkit
    TilaJulkaistu - 2018
    OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
    TapahtumaJoint Conference of the European Medical and Biological Engineering Conference (EMBEC) and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC) -
    Kesto: 1 tammik. 1900 → …

    Julkaisusarja

    NimiIFMBE Proceedings
    Vuosikerta65
    ISSN (painettu)1680-0737

    Conference

    ConferenceJoint Conference of the European Medical and Biological Engineering Conference (EMBEC) and the Nordic-Baltic Conference on Biomedical Engineering and Medical Physics (NBC)
    Ajanjakso1/01/00 → …

    Julkaisufoorumi-taso

    • Jufo-taso 1

    !!ASJC Scopus subject areas

    • Biomedical Engineering
    • Bioengineering

    Sormenjälki

    Sukella tutkimusaiheisiin 'Multiresolution MAPEM method for 3D reconstruction of symmetrical particles with electron microscopy'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.

    Siteeraa tätä