Oxygen-induced retinopathy model for ischemic retinal diseases in rodents

Maria Vähätupa, Niina Jääskeläinen, Marc Cerrada-Gimenez, Rubina Thapa, Tero Järvinen, Giedrius Kalesnykas, Hannele Uusitalo-Järvinen

Tutkimustuotos: ArtikkeliScientificvertaisarvioitu

2 Sitaatiot (Scopus)

Abstrakti

One of the commonly used models for ischemic retinopathies is the oxygen-induced retinopathy (OIR) model. Here we describe detailed protocols for the OIR model induction and its readouts in both mice and rats. Retinal neovascularization is induced in OIR by exposing rodent pups either to hyperoxia (mice) or alternating levels of hyperoxia and hypoxia (rats). The primary readouts of these models are the size of neovascular (NV) and avascular (AVA) areas in the retina. This preclinical in vivo model can be used to evaluate the efficacy of potential anti-angiogenic drugs or to address the role of specific genes in the retinal angiogenesis by using genetically manipulated animals. The model has some strain and vendor specific variation in the OIR induction which should be taken into consideration when designing the experiments.

AlkuperäiskieliEnglanti
Artikkelie61482
Sivumäärä19
JulkaisuJournal of Visualized Experiments
Vuosikerta2020
Numero163
DOI - pysyväislinkit
TilaJulkaistu - 2020
OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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