Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy

Joose Kreutzer; Marlitt Viehrig; Antti-Juhana Maki; Pasi Kallio; Rolle Rahikainen; Vesa Hytönen

doi:10.1109/marss.2017.8001929

Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy

Joose Kreutzer
, Marlitt Viehrig
, Antti-Juhana Maki
, Pasi Kallio
, Rolle Rahikainen
, Vesa Hytönen

Tutkimustuotos: Konferenssiartikkeli › Tieteellinen › vertaisarvioitu

3 Sitaatiot (Scopus)

53 Lataukset (Pure)

Abstrakti

In this study, we demonstrate the functionality and usability of a compact, pneumatically actuated, elastomeric stimulation device for mechanobiological studies. The soft mechatronic device enables high-resolution live-cell confocal fluorescent imaging during equiaxial stretching. Several single cells can be tracked and imaged repeatedly after stretching periods. For demonstration, we provide image based analysis of dynamic change of the cell body and the nucleus area and actin fiber orientation during mechanical stimulation of mouse embryonic fibroblast (MEF) cells. Additionally, we present the characteristics of the device utilizing computational simulations and experimental validation using a particle tracking method for strain field analysis.

Alkuperäiskieli	Englanti
Otsikko	International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings
Toimittajat	S Haliyo, S Martel, S. Fatikow
Kustantaja	IEEE
ISBN (elektroninen)	978-1-5386-0346-8
DOI - pysyväislinkit	https://doi.org/10.1109/marss.2017.8001929
Tila	Julkaistu - 2017
OKM-julkaisutyyppi	A4 Artikkeli konferenssijulkaisussa
Tapahtuma	International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS) - Kesto: 1 tammik. 2017 → …

Conference

Conference	International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS)
Ajanjakso	1/01/17 → …

Tutkimusalat

High-resolution imaging
Mechanical stimulation
Mouse embryonic fibroblasts
PDMS
Particle tracking
Pneumatic actuation
Strain field analysis

Julkaisufoorumi-taso

Jufo-taso 1

Pääsy asiakirjaan

10.1109/marss.2017.8001929

MARSS2017 - KreutzerAccepted author manuscript, 1,94 MB

http://urn.fi/urn:nbn:fi:tty-201712202442

Siteeraa tätä

Kreutzer, J., Viehrig, M., Maki, A.-J., Kallio, P., Rahikainen, R., & Hytönen, V. (2017). Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy. teoksessa S. Haliyo, S. Martel, & S. Fatikow (Toimittajat), International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings IEEE. https://doi.org/10.1109/marss.2017.8001929

@inproceedings{265b6293de11403cb5e9f5a099250e40,

title = "Pneumatically actuated elastomeric device for simultaneous mechanobiological studies \& live-cell fluorescent microscopy",

abstract = "In this study, we demonstrate the functionality and usability of a compact, pneumatically actuated, elastomeric stimulation device for mechanobiological studies. The soft mechatronic device enables high-resolution live-cell confocal fluorescent imaging during equiaxial stretching. Several single cells can be tracked and imaged repeatedly after stretching periods. For demonstration, we provide image based analysis of dynamic change of the cell body and the nucleus area and actin fiber orientation during mechanical stimulation of mouse embryonic fibroblast (MEF) cells. Additionally, we present the characteristics of the device utilizing computational simulations and experimental validation using a particle tracking method for strain field analysis.",

keywords = "High-resolution imaging, Mechanical stimulation, Mouse embryonic fibroblasts, PDMS, Particle tracking, Pneumatic actuation, Strain field analysis, High-resolution imaging, Mechanical stimulation, Mouse embryonic fibroblasts, PDMS, Particle tracking, Pneumatic actuation, Strain field analysis",

author = "Joose Kreutzer and Marlitt Viehrig and Antti-Juhana Maki and Pasi Kallio and Rolle Rahikainen and Vesa Hyt{\"o}nen",

note = "INT=tut-bmt,{"}Viehrig, Marlitt{"}; International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS) ; Conference date: 01-01-2017",

year = "2017",

doi = "10.1109/marss.2017.8001929",

language = "English",

editor = "S Haliyo and S Martel and S. Fatikow",

booktitle = "International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings",

publisher = "IEEE",

address = "United States",

}

Kreutzer, J, Viehrig, M, Maki, A-J, Kallio, P , Rahikainen, R & Hytönen, V 2017, Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy. julkaisussa S Haliyo, S Martel & S Fatikow (toim), International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings. IEEE, International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS), 1/01/17. https://doi.org/10.1109/marss.2017.8001929

Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy. / Kreutzer, Joose; Viehrig, Marlitt; Maki, Antti-Juhana et al.
International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings. toim. / S Haliyo; S Martel; S. Fatikow. IEEE, 2017.

Tutkimustuotos: Konferenssiartikkeli › Tieteellinen › vertaisarvioitu

TY - GEN

T1 - Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy

AU - Kreutzer, Joose

AU - Viehrig, Marlitt

AU - Maki, Antti-Juhana

AU - Kallio, Pasi

AU - Rahikainen, Rolle

AU - Hytönen, Vesa

N1 - INT=tut-bmt,"Viehrig, Marlitt"

PY - 2017

Y1 - 2017

N2 - In this study, we demonstrate the functionality and usability of a compact, pneumatically actuated, elastomeric stimulation device for mechanobiological studies. The soft mechatronic device enables high-resolution live-cell confocal fluorescent imaging during equiaxial stretching. Several single cells can be tracked and imaged repeatedly after stretching periods. For demonstration, we provide image based analysis of dynamic change of the cell body and the nucleus area and actin fiber orientation during mechanical stimulation of mouse embryonic fibroblast (MEF) cells. Additionally, we present the characteristics of the device utilizing computational simulations and experimental validation using a particle tracking method for strain field analysis.

AB - In this study, we demonstrate the functionality and usability of a compact, pneumatically actuated, elastomeric stimulation device for mechanobiological studies. The soft mechatronic device enables high-resolution live-cell confocal fluorescent imaging during equiaxial stretching. Several single cells can be tracked and imaged repeatedly after stretching periods. For demonstration, we provide image based analysis of dynamic change of the cell body and the nucleus area and actin fiber orientation during mechanical stimulation of mouse embryonic fibroblast (MEF) cells. Additionally, we present the characteristics of the device utilizing computational simulations and experimental validation using a particle tracking method for strain field analysis.

KW - High-resolution imaging

KW - Mechanical stimulation

KW - Mouse embryonic fibroblasts

KW - PDMS

KW - Particle tracking

KW - Pneumatic actuation

KW - Strain field analysis

KW - High-resolution imaging

KW - Mechanical stimulation

KW - Mouse embryonic fibroblasts

KW - PDMS

KW - Particle tracking

KW - Pneumatic actuation

KW - Strain field analysis

U2 - 10.1109/marss.2017.8001929

DO - 10.1109/marss.2017.8001929

M3 - Conference contribution

AN - SCOPUS:85030222654

BT - International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings

A2 - Haliyo, S

A2 - Martel, S

A2 - Fatikow, S.

PB - IEEE

T2 - International Conference on Manipulation, Automation and Robotics at Small Scales (MARSS)

Y2 - 1 January 2017

ER -

Kreutzer J, Viehrig M, Maki AJ, Kallio P , Rahikainen R , Hytönen V. Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy. julkaisussa Haliyo S, Martel S, Fatikow S, toimittajat, International Conference on Manipulation, Automation and Robotics at Small Scales, MARSS 2017 - Proceedings. IEEE. 2017 doi: 10.1109/marss.2017.8001929

Pneumatically actuated elastomeric device for simultaneous mechanobiological studies & live-cell fluorescent microscopy

Abstrakti

Conference

Tutkimusalat

Julkaisufoorumi-taso

Pääsy asiakirjaan

Sormenjälki

Siteeraa tätä