Production and characterization of virus-like particles and the P domain protein of GII.4 norovirus

Tiia Koho, Leena Huhti, Vesna Blazevic, Kirsi Nurminen, Sarah J. Butcher, Pasi Laurinmäki, Nisse Kalkkinen, Gunilla Rönnholm, Timo Vesikari, Vesa P. Hytönen, Markku S. Kulomaa

    Tutkimustuotos: ArtikkeliTieteellinenvertaisarvioitu

    38 Sitaatiot (Scopus)

    Abstrakti

    Noroviruses are an important cause of epidemic acute gastroenteritis in humans. In this study the production and characterization of GII.4 norovirus virus-like particles (VLPs) in insect cells is reported. Furthermore, the expression of corresponding norovirus polyhistidine-tagged P domain protein in Escherichia coli is described. The protruding P domain of the norovirus capsid is known to contain determinants for antibody and receptor binding. Therefore, P domain proteins were studied as an alternative diagnostic tool for evaluating norovirus infection. Analyses by dynamic light scattering and cryo-electron microscopy revealed the presence of intact VLPs with an average diameter of about 40. nm. Immunostaining and ELISA assays using norovirus-specific human sera revealed that VLPs and the P domain are recognized by norovirus-specific antibodies and by their putative receptor. The VLPs and P domain protein are potentially useful in the development of diagnostic and vaccination tools for noroviruses.

    AlkuperäiskieliEnglanti
    Sivut1-7
    Sivumäärä7
    JulkaisuJOURNAL OF VIROLOGICAL METHODS
    Vuosikerta179
    Numero1
    DOI - pysyväislinkit
    TilaJulkaistu - 2012
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

    Tutkimusalat

    • Diagnostics
    • Norovirus
    • P domain protein
    • VLP
    • Virus-like particle

    Julkaisufoorumi-taso

    • Jufo-taso 1

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