Production of l-xylose from l-xylulose using Escherichia coli l-fucose isomerase

Anne Usvalampi, Ossi Turunen, Jarkko Valjakka, Ossi Pastinen, Matti Leisola, Antti Nyyssölä

    Tutkimustuotos: ArtikkeliScientificvertaisarvioitu

    11 Sitaatiot (Scopus)

    Abstrakti

    l-Xylulose was used as a raw material for the production of l-xylose with a recombinantly produced Escherichia coli l-fucose isomerase as the catalyst. The enzyme had a very alkaline pH optimum (over 10.5) and displayed Michaelis-Menten kinetics for l-xylulose with a K m of 41mM and a V max of 0.23μmol/(mgmin). The half-lives determined for the enzyme at 35°C and at 45°C were 6h 50min and 1h 31min, respectively. The reaction equilibrium between l-xylulose and l-xylose was 15:85 at 35°C and thus favored the formation of l-xylose. Contrary to the l-rhamnose isomerase catalyzed reaction described previously [14] l-lyxose was not detected in the reaction mixture with l-fucose isomerase. Although xylitol acted as an inhibitor of the reaction, even at a high ratio of xylitol to l-xylulose the inhibition did not reach 50%.

    AlkuperäiskieliEnglanti
    Sivut71-76
    Sivumäärä6
    JulkaisuENZYME AND MICROBIAL TECHNOLOGY
    Vuosikerta50
    Numero1
    DOI - pysyväislinkit
    TilaJulkaistu - 2012
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

    Tutkimusalat

    • Isomerization
    • L-Fucose isomerase
    • L-Xylose
    • L-Xylulose
    • Rare sugars
    • l-Fucose isomerase
    • l-Xylose
    • l-Xylulose

    Julkaisufoorumi-taso

    • Jufo-taso 1

    Sormenjälki

    Sukella tutkimusaiheisiin 'Production of l-xylose from l-xylulose using Escherichia coli l-fucose isomerase'. Ne muodostavat yhdessä ainutlaatuisen sormenjäljen.

    Siteeraa tätä