Role of HLA-DQ typing and antitissue transglutaminase antibody titres in diagnosing coeliac disease among Sudanese children with type 1 diabetes mellitus

Ibtihag Ibaid; Mohammed Hussien; Katri Kaukinen; Omayma Sabir; Miskelyemen Elmekki; Azza Musa; Nasreldein Abdelhadi; Abdel Rahim El Hussein; Päivi Saavalainen

doi:10.1136/bmjgast-2021-000735

Role of HLA-DQ typing and antitissue transglutaminase antibody titres in diagnosing coeliac disease among Sudanese children with type 1 diabetes mellitus

Ibtihag Ibaid^*
, Mohammed Hussien
, Katri Kaukinen
, Omayma Sabir
, Miskelyemen Elmekki
, Azza Musa
, Nasreldein Abdelhadi
, Abdel Rahim El Hussein
, Päivi Saavalainen

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Artikkeli › Tieteellinen › vertaisarvioitu

5 Sitaatiot (Scopus)

21 Lataukset (Pure)

Abstrakti

Objective The aim of the study was to determine the prevalence of coeliac disease (CD) and to recognise Human leukocyte antigen (HLA)-associated hereditary susceptibility to Sudanese CD patients with type 1 diabetes mellitus (DM1). Design Antitissue transglutaminase IgA (anti-TG IgA) was measured in the serum of 373 children affected with DM1 aged 1-19-year old and in 100 serum samples from non-diabetic control children. Histological examination was performed in 19 children seropositive for anti-TG IgA (17 DMI and 2 controls). Additionally, PCR-based analysis of Major histocompatibility complex, class II, DQ beta 1 (HLA-DQB1) genotyping was implemented in three study population groups as follows: group 1 (n=25) (+ve DM1 and +ve CD), group 2 (n=63) (-ve DM1 and +ve CD) and control group 3 (n=2) (+ve CD). Results Twenty-six Sudanese children with DM1 out of 373 (6.97%) were seropositive for anti-TG IgA. Duodenal biopsy revealed Marsh 2 and 3 in 13 out of 17 (76.47%) seropositive anti-TG IgA patients with DM1. Significant association (p<0.05) was detected between the level of anti-TG IgA autoantibodies (IU/mL) and Marsh stage. HLA DQ2 and DQ8 were found in 88% (22/25) and 8% (2/25) of examined patients with CD with DM1, respectively. Conclusions Anti-TG IgA titre of greater than 10 times upper limit of normal (≥10× ULN) can be useful for detecting CD in children with type 1 diabetes without duodenal biopsy. HLA testing in children with DM1 appears to provide little added benefit given the high prevalence (96%) of HLA DQ2/DQ8 in children with DM1.

Alkuperäiskieli	Englanti
Artikkeli	e000735
Julkaisu	BMJ Open Gastroenterology
Vuosikerta	9
Numero	1
DOI - pysyväislinkit	https://doi.org/10.1136/bmjgast-2021-000735
Tila	Julkaistu - 24 tammik. 2022
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

Rahoitus

Funding This study was supported by the Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan (Grant Number 2007/152). 7Department of Statistics and Documentation, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan 8Faculty of Pharmacy, The National Ribat University, Khartoum, Sudan, Khartoum, Sudan 9Deparment of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan 10Translational Immunology Research Program, Department of Clinical and Medical Genetics, University of Helsinki, Helsinki, Finland Contributors II and MH carried out the methodology and drafted the manuscript. OS provided the samples and data collection. AM conducted the statistical analysis. NA, KK, AREH and PS contributed to the conception and design of the study and revised the manuscript. II acting as guarator. All authors have read and approved the final version of this manuscript.

YK:n kestävän kehityksen tavoitteet

Tämä tuotos edistää seuraavia kestävän kehityksen tavoitteita:

SDG 3 – Hyvä terveys ja hyvinvointi

Julkaisufoorumi-taso

Jufo-taso 1

!!ASJC Scopus subject areas

Gastroenterology

Pääsy asiakirjaan

10.1136/bmjgast-2021-000735Lisenssi: CC BY-NC

e000735.fullFinal published version, 242 KBLisenssi: CC BY-NC

https://urn.fi/URN:NBN:fi:tuni-202204294158Lisenssi: CC BY-NC

Siteeraa tätä

Ibaid, I., Hussien, M., Kaukinen, K., Sabir, O., Elmekki, M., Musa, A., Abdelhadi, N., El Hussein, A. R., & Saavalainen, P. (2022). Role of HLA-DQ typing and antitissue transglutaminase antibody titres in diagnosing coeliac disease among Sudanese children with type 1 diabetes mellitus. BMJ Open Gastroenterology, 9(1), Artikkeli e000735. https://doi.org/10.1136/bmjgast-2021-000735

@article{aa718ec36e1f460e91c85eb8f991fa61,

title = "Role of HLA-DQ typing and antitissue transglutaminase antibody titres in diagnosing coeliac disease among Sudanese children with type 1 diabetes mellitus",

abstract = "Objective The aim of the study was to determine the prevalence of coeliac disease (CD) and to recognise Human leukocyte antigen (HLA)-associated hereditary susceptibility to Sudanese CD patients with type 1 diabetes mellitus (DM1). Design Antitissue transglutaminase IgA (anti-TG IgA) was measured in the serum of 373 children affected with DM1 aged 1-19-year old and in 100 serum samples from non-diabetic control children. Histological examination was performed in 19 children seropositive for anti-TG IgA (17 DMI and 2 controls). Additionally, PCR-based analysis of Major histocompatibility complex, class II, DQ beta 1 (HLA-DQB1) genotyping was implemented in three study population groups as follows: group 1 (n=25) (+ve DM1 and +ve CD), group 2 (n=63) (-ve DM1 and +ve CD) and control group 3 (n=2) (+ve CD). Results Twenty-six Sudanese children with DM1 out of 373 (6.97\%) were seropositive for anti-TG IgA. Duodenal biopsy revealed Marsh 2 and 3 in 13 out of 17 (76.47\%) seropositive anti-TG IgA patients with DM1. Significant association (p<0.05) was detected between the level of anti-TG IgA autoantibodies (IU/mL) and Marsh stage. HLA DQ2 and DQ8 were found in 88\% (22/25) and 8\% (2/25) of examined patients with CD with DM1, respectively. Conclusions Anti-TG IgA titre of greater than 10 times upper limit of normal (≥10× ULN) can be useful for detecting CD in children with type 1 diabetes without duodenal biopsy. HLA testing in children with DM1 appears to provide little added benefit given the high prevalence (96\%) of HLA DQ2/DQ8 in children with DM1. ",

keywords = "celiac disease, nutrition, paediatric gastroenterology",

author = "Ibtihag Ibaid and Mohammed Hussien and Katri Kaukinen and Omayma Sabir and Miskelyemen Elmekki and Azza Musa and Nasreldein Abdelhadi and \{El Hussein\}, \{Abdel Rahim\} and P{\"a}ivi Saavalainen",

note = "Funding Information: Funding This study was supported by the Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan (Grant Number 2007/152). Funding Information: 7Department of Statistics and Documentation, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan 8Faculty of Pharmacy, The National Ribat University, Khartoum, Sudan, Khartoum, Sudan 9Deparment of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan 10Translational Immunology Research Program, Department of Clinical and Medical Genetics, University of Helsinki, Helsinki, Finland Contributors II and MH carried out the methodology and drafted the manuscript. OS provided the samples and data collection. AM conducted the statistical analysis. NA, KK, AREH and PS contributed to the conception and design of the study and revised the manuscript. II acting as guarator. All authors have read and approved the final version of this manuscript. Publisher Copyright: {\textcopyright} Author(s) (or their employer(s)) 2022.",

year = "2022",

month = jan,

day = "24",

doi = "10.1136/bmjgast-2021-000735",

language = "English",

volume = "9",

journal = "BMJ Open Gastroenterology",

issn = "2054-4774",

publisher = "BMJ Publishing Group",

number = "1",

}

Ibaid, I, Hussien, M, Kaukinen, K, Sabir, O, Elmekki, M, Musa, A, Abdelhadi, N, El Hussein, AR & Saavalainen, P 2022, 'Role of HLA-DQ typing and antitissue transglutaminase antibody titres in diagnosing coeliac disease among Sudanese children with type 1 diabetes mellitus', BMJ Open Gastroenterology, Vuosikerta 9, Nro 1, e000735. https://doi.org/10.1136/bmjgast-2021-000735

TY - JOUR

T1 - Role of HLA-DQ typing and antitissue transglutaminase antibody titres in diagnosing coeliac disease among Sudanese children with type 1 diabetes mellitus

AU - Ibaid, Ibtihag

AU - Hussien, Mohammed

AU - Kaukinen, Katri

AU - Sabir, Omayma

AU - Elmekki, Miskelyemen

AU - Musa, Azza

AU - Abdelhadi, Nasreldein

AU - El Hussein, Abdel Rahim

AU - Saavalainen, Päivi

N1 - Funding Information: Funding This study was supported by the Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan (Grant Number 2007/152). Funding Information: 7Department of Statistics and Documentation, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan 8Faculty of Pharmacy, The National Ribat University, Khartoum, Sudan, Khartoum, Sudan 9Deparment of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan 10Translational Immunology Research Program, Department of Clinical and Medical Genetics, University of Helsinki, Helsinki, Finland Contributors II and MH carried out the methodology and drafted the manuscript. OS provided the samples and data collection. AM conducted the statistical analysis. NA, KK, AREH and PS contributed to the conception and design of the study and revised the manuscript. II acting as guarator. All authors have read and approved the final version of this manuscript. Publisher Copyright: © Author(s) (or their employer(s)) 2022.

PY - 2022/1/24

Y1 - 2022/1/24

N2 - Objective The aim of the study was to determine the prevalence of coeliac disease (CD) and to recognise Human leukocyte antigen (HLA)-associated hereditary susceptibility to Sudanese CD patients with type 1 diabetes mellitus (DM1). Design Antitissue transglutaminase IgA (anti-TG IgA) was measured in the serum of 373 children affected with DM1 aged 1-19-year old and in 100 serum samples from non-diabetic control children. Histological examination was performed in 19 children seropositive for anti-TG IgA (17 DMI and 2 controls). Additionally, PCR-based analysis of Major histocompatibility complex, class II, DQ beta 1 (HLA-DQB1) genotyping was implemented in three study population groups as follows: group 1 (n=25) (+ve DM1 and +ve CD), group 2 (n=63) (-ve DM1 and +ve CD) and control group 3 (n=2) (+ve CD). Results Twenty-six Sudanese children with DM1 out of 373 (6.97%) were seropositive for anti-TG IgA. Duodenal biopsy revealed Marsh 2 and 3 in 13 out of 17 (76.47%) seropositive anti-TG IgA patients with DM1. Significant association (p<0.05) was detected between the level of anti-TG IgA autoantibodies (IU/mL) and Marsh stage. HLA DQ2 and DQ8 were found in 88% (22/25) and 8% (2/25) of examined patients with CD with DM1, respectively. Conclusions Anti-TG IgA titre of greater than 10 times upper limit of normal (≥10× ULN) can be useful for detecting CD in children with type 1 diabetes without duodenal biopsy. HLA testing in children with DM1 appears to provide little added benefit given the high prevalence (96%) of HLA DQ2/DQ8 in children with DM1.

AB - Objective The aim of the study was to determine the prevalence of coeliac disease (CD) and to recognise Human leukocyte antigen (HLA)-associated hereditary susceptibility to Sudanese CD patients with type 1 diabetes mellitus (DM1). Design Antitissue transglutaminase IgA (anti-TG IgA) was measured in the serum of 373 children affected with DM1 aged 1-19-year old and in 100 serum samples from non-diabetic control children. Histological examination was performed in 19 children seropositive for anti-TG IgA (17 DMI and 2 controls). Additionally, PCR-based analysis of Major histocompatibility complex, class II, DQ beta 1 (HLA-DQB1) genotyping was implemented in three study population groups as follows: group 1 (n=25) (+ve DM1 and +ve CD), group 2 (n=63) (-ve DM1 and +ve CD) and control group 3 (n=2) (+ve CD). Results Twenty-six Sudanese children with DM1 out of 373 (6.97%) were seropositive for anti-TG IgA. Duodenal biopsy revealed Marsh 2 and 3 in 13 out of 17 (76.47%) seropositive anti-TG IgA patients with DM1. Significant association (p<0.05) was detected between the level of anti-TG IgA autoantibodies (IU/mL) and Marsh stage. HLA DQ2 and DQ8 were found in 88% (22/25) and 8% (2/25) of examined patients with CD with DM1, respectively. Conclusions Anti-TG IgA titre of greater than 10 times upper limit of normal (≥10× ULN) can be useful for detecting CD in children with type 1 diabetes without duodenal biopsy. HLA testing in children with DM1 appears to provide little added benefit given the high prevalence (96%) of HLA DQ2/DQ8 in children with DM1.

KW - celiac disease

KW - nutrition

KW - paediatric gastroenterology

U2 - 10.1136/bmjgast-2021-000735

DO - 10.1136/bmjgast-2021-000735

M3 - Article

AN - SCOPUS:85124072747

SN - 2054-4774

VL - 9

JO - BMJ Open Gastroenterology

JF - BMJ Open Gastroenterology

IS - 1

M1 - e000735

ER -

Role of HLA-DQ typing and antitissue transglutaminase antibody titres in diagnosing coeliac disease among Sudanese children with type 1 diabetes mellitus

Abstrakti

Rahoitus

YK:n kestävän kehityksen tavoitteet

Julkaisufoorumi-taso

!!ASJC Scopus subject areas

Pääsy asiakirjaan

Sormenjälki

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