Simulation of developing human neuronal cell networks

Kerstin Lenk, Barbara Priwitzer, Laura Ylä-Outinen, Lukas H B Tietz, Susanna Narkilahti, Jari A.K. Hyttinen

    Tutkimustuotos: ArticleScientificvertaisarvioitu

    2 Sitaatiot (Scopus)
    29 Lataukset (Pure)

    Abstrakti

    Background: Microelectrode array (MEA) is a widely used technique to study for example the functional properties of neuronal networks derived from human embryonic stem cells (hESC-NN). With hESC-NN, we can investigate the earliest developmental stages of neuronal network formation in the human brain. Methods: In this paper, we propose an in silico model of maturating hESC-NNs based on a phenomenological model called INEX. We focus on simulations of the development of bursts in hESC-NNs, which are the main feature of neuronal activation patterns. The model was developed with data from developing hESC-NN recordings on MEAs which showed increase in the neuronal activity during the investigated six measurement time points in the experimental and simulated data. Results: Our simulations suggest that the maturation process of hESC-NN, resulting in the formation of bursts, can be explained by the development of synapses. Moreover, spike and burst rate both decreased at the last measurement time point suggesting a pruning of synapses as the weak ones are removed. Conclusions: To conclude, our model reflects the assumption that the interaction between excitatory and inhibitory neurons during the maturation of a neuronal network and the spontaneous emergence of bursts are due to increased connectivity caused by the forming of new synapses.

    AlkuperäiskieliEnglanti
    Artikkeli105
    JulkaisuBioMedical Engineering Online
    Vuosikerta15
    Numero1
    DOI - pysyväislinkit
    TilaJulkaistu - 2016
    OKM-julkaisutyyppiA1 Alkuperäisartikkeli

    Tutkimusalat

    • Development
    • Human embryonic stem cells
    • Microelectrode array
    • Neuronal networks
    • Simulation

    Julkaisufoorumi-taso

    • Jufo-taso 1

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