TY - JOUR
T1 - Surface modification of silicate, borosilicate and phosphate bioactive glasses to improve/control protein adsorption
T2 - PART I
AU - Gobbo, Virginia Alessandra
AU - Parihar, Vijay Singh
AU - Prato, Mirko
AU - Kellomäki, Minna
AU - Vernè, Enrica
AU - Spriano, Silvia
AU - Massera, Jonathan
N1 - Funding Information:
The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Virginia Alessandra Gobbo reports financial support was provided by Horizon 2020. Virginia Alessandra Gobbo reports a relationship with Horizon 2020 that includes: funding grants.This work was funded by the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement [No 860462 (PREMUROSA)]. The authors would also like to acknowledge Tampere Microscopy Center for the processing and analysis of the SEM/EDS data.
Funding Information:
This work was funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement [No 860462 (PREMUROSA)]. The authors would also like to acknowledge Tampere Microscopy Center for the processing and analysis of the SEM/EDS data.
Funding Information:
The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Virginia Alessandra Gobbo reports financial support was provided by Horizon 2020 . Virginia Alessandra Gobbo reports a relationship with Horizon 2020 that includes: funding grants.
Publisher Copyright:
© 2022 The Authors
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Bioactive glasses (BGs) are promising for bone tissue regeneration. BG composition can be tailored, according to the application of interest, and/or functionalized with organic molecules/biomolecules to improve their performances. However, despite the wide knowledge concerning BGs, their interaction with proteins, fundamental for controlling the fate of the implant, has not been deeply investigated yet. Controlling or predicting protein adsorption requires a full understanding of the materials surface physico-chemical properties. In this work, four different BGs (S53P4, B25, SCNB, PhGlass) were surface-modified by four different treatments: 72 h-soaking in TRIS, 72 h soaking in simulated body fluid, APTES grafting and quaternized APTES grafting. The surfaces were then characterized both untreated and after each treatment by contact angle, zeta potential analysis, X-ray photoelectron spectroscopy, Fourier Transform InfraRed–Attenuated Total Reflectance spectroscopy and Scanning Electron Microscopy and Energy Dispersive Spectroscopy. Inductively Coupled Plasma – Optical Emission Spectrometry was then performed to investigate the ion leaching. The aim of this study (Part I) is the physico-chemical characterization of BGs as a function of the implemented treatments, aiming to better understand how the superficial properties are successively affecting protein adsorption. Protein adsorption on untreated and treated BGs will be discussed in a following manuscript (Part II).
AB - Bioactive glasses (BGs) are promising for bone tissue regeneration. BG composition can be tailored, according to the application of interest, and/or functionalized with organic molecules/biomolecules to improve their performances. However, despite the wide knowledge concerning BGs, their interaction with proteins, fundamental for controlling the fate of the implant, has not been deeply investigated yet. Controlling or predicting protein adsorption requires a full understanding of the materials surface physico-chemical properties. In this work, four different BGs (S53P4, B25, SCNB, PhGlass) were surface-modified by four different treatments: 72 h-soaking in TRIS, 72 h soaking in simulated body fluid, APTES grafting and quaternized APTES grafting. The surfaces were then characterized both untreated and after each treatment by contact angle, zeta potential analysis, X-ray photoelectron spectroscopy, Fourier Transform InfraRed–Attenuated Total Reflectance spectroscopy and Scanning Electron Microscopy and Energy Dispersive Spectroscopy. Inductively Coupled Plasma – Optical Emission Spectrometry was then performed to investigate the ion leaching. The aim of this study (Part I) is the physico-chemical characterization of BGs as a function of the implemented treatments, aiming to better understand how the superficial properties are successively affecting protein adsorption. Protein adsorption on untreated and treated BGs will be discussed in a following manuscript (Part II).
KW - Bioactive glasses
KW - Surface charge
KW - Surface modification
KW - Surface physico-chemical properties
KW - Surface reaction
U2 - 10.1016/j.ceramint.2022.09.105
DO - 10.1016/j.ceramint.2022.09.105
M3 - Article
AN - SCOPUS:85138765563
SN - 0272-8842
VL - 49
SP - 1261
EP - 1275
JO - Ceramics International
JF - Ceramics International
IS - 1
ER -