Synchrony between Genetic Repressilators in Sister Cells in Different Temperatures

Jerome G. Chandraseelan, Samuel M.D. Oliveira, Antti Häkkinen, Sofia Startceva, Andre S. Ribeiro

    Tutkimustuotos: KonferenssiartikkeliScientificvertaisarvioitu

    Abstrakti

    We used live E. coli containing synthetic genetic oscillators to study how the degree of synchrony between the genetic circuits of sister cells changes with temperature. We found that both the mean and the variability of the degree of synchrony between the fluorescence signals from sister cells are affected by temperature. Also, while most pairs of sister cells were found to be highly synchronous in each condition, the number of asynchronous pairs increased with increasing temperature, which was found to be due to disruptions in the oscillations. Finally we provide evidence that these disruptions tend to affect multiple generations as opposed to individual cells. These findings provide insight in how to design more robust synthetic circuits and in how cell division can affect their dynamics.
    AlkuperäiskieliEnglanti
    OtsikkoICCMSB 2015: International Conference on Computational and Molecular Systems Biology
    KustantajaWorld Academy of Science, Engineering and Technology
    Sivut725-728
    Sivumäärä4
    TilaJulkaistu - 2015
    OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
    TapahtumaInternational Conference on Computational and Molecular Systems Biology - , Iso-Britannia
    Kesto: 1 tammik. 2015 → …

    Julkaisusarja

    NimiProceedings of the World Academy of Science, Engineering and Technology
    ISSN (painettu)2010-376X
    ISSN (elektroninen)2010-3778

    Conference

    ConferenceInternational Conference on Computational and Molecular Systems Biology
    Maa/AlueIso-Britannia
    Ajanjakso1/01/15 → …

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