Temperature dependence of leakiness of transcription repression mechanisms of Escherichia coli

Nadia Goncalves, Samuel M D Oliveira, Vinodh K. Kandavalli, Jose M. Fonseca, Andre S. Ribeiro

    Tutkimustuotos: KonferenssiartikkeliScientificvertaisarvioitu

    2 Sitaatiot (Scopus)

    Abstrakti

    In E. coli, transcription repression is essential in cellular functioning. However, its failure rates are non-negligible. We measured the leakiness rate of lacO3O1 promoter with single RNA sensitivity and its temperature dependence in live cells. After finding strong temperature dependence, we dissected the causes. While RNA polymerase numbers and kt, the rate of active transcription, vary weakly with temperature, the repression strength (dependent on number of repressors and binding and unbinding rates of repressors to the promoter) is heavily temperature dependent. We conclude that the lacO3O1 leakiness at low temperatures increases as the repression mechanism’s efficiency hampers.

    AlkuperäiskieliEnglanti
    OtsikkoComputational Methods in Systems Biology - 14th International Conference, CMSB 2016
    KustantajaSpringer Verlag
    Sivut341-342
    Sivumäärä2
    ISBN (painettu)9783319451763
    TilaJulkaistu - 2016
    OKM-julkaisutyyppiA4 Artikkeli konferenssijulkaisussa
    TapahtumaInternational Conference on Computational Methods in Systems Biology -
    Kesto: 1 tammik. 1900 → …

    Julkaisusarja

    NimiLecture Notes in Computer Science
    Vuosikerta9859
    ISSN (painettu)0302-9743
    ISSN (elektroninen)1611-3349

    Conference

    ConferenceInternational Conference on Computational Methods in Systems Biology
    Ajanjakso1/01/00 → …

    Julkaisufoorumi-taso

    • Jufo-taso 1

    !!ASJC Scopus subject areas

    • Theoretical Computer Science
    • Computer Science(all)

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