Visual Counting and Automated Image-analytic Assessment of Ki-67 and their Prognostic Value in Synovial Sarcoma

Riikka E Laurila; Tom O Böhling; Carl P Blomqvist; Christina Karlsson; Erkki J Tukiainen; Jussi Repo; Mika M Sampo

doi:10.21873/cdp.10070

Visual Counting and Automated Image-analytic Assessment of Ki-67 and their Prognostic Value in Synovial Sarcoma

Riikka E Laurila, Tom O Böhling, Carl P Blomqvist, Christina Karlsson, Erkki J Tukiainen, Jussi Repo, Mika M Sampo

Tutkimustuotos: Artikkeli › Scientific › vertaisarvioitu

5 Lataukset (Pure)

Abstrakti

BACKGROUND: Ki-67 is a widely used proliferation marker reflecting prognosis in various tumors. However, visual assessment and scoring of Ki-67 suffers from marked inter-observer and intra-observer variability. We aimed to assess the concordance of manual counting and automated image-analytic scoring methods for Ki-67 in synovial sarcoma.

PATIENTS AND METHODS: Tissue microarrays from 34 patients with synovial sarcoma were immunostained for Ki-67 and scored both visually and with 3DHistech QuantCenter.

RESULTS: The automated assessment of Ki-67 expression was in good agreement with the visually counted Ki-67 (r Pearson =0.96, p<0.001). In a Cox regression model automated [hazard ratio (HR)=1.047, p=0.024], but not visual (HR=1.063, p=0.053) assessment method associated high Ki-67 scores with worse overall survival.

CONCLUSION: The automated Ki-67 assessment method appears to be comparable to the visual method in synovial sarcoma and had a significant association to overall survival.

Alkuperäiskieli	Englanti
Sivut	7-14
Sivumäärä	8
Julkaisu	Cancer diagnosis & prognosis
Vuosikerta	2
Numero	1
DOI - pysyväislinkit	https://doi.org/10.21873/cdp.10070
Tila	Julkaistu - 2022
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä

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10.21873/cdp.10070

7Final published version, 2,36 MBLisenssi: CC BY-NC-ND

https://urn.fi/URN:NBN:fi:tuni-202302032032Lisenssi: CC BY-NC-ND

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@article{5a9b8177992944178c9f6530229a9c21,

title = "Visual Counting and Automated Image-analytic Assessment of Ki-67 and their Prognostic Value in Synovial Sarcoma",

abstract = "BACKGROUND: Ki-67 is a widely used proliferation marker reflecting prognosis in various tumors. However, visual assessment and scoring of Ki-67 suffers from marked inter-observer and intra-observer variability. We aimed to assess the concordance of manual counting and automated image-analytic scoring methods for Ki-67 in synovial sarcoma.PATIENTS AND METHODS: Tissue microarrays from 34 patients with synovial sarcoma were immunostained for Ki-67 and scored both visually and with 3DHistech QuantCenter.RESULTS: The automated assessment of Ki-67 expression was in good agreement with the visually counted Ki-67 (r Pearson =0.96, p<0.001). In a Cox regression model automated [hazard ratio (HR)=1.047, p=0.024], but not visual (HR=1.063, p=0.053) assessment method associated high Ki-67 scores with worse overall survival.CONCLUSION: The automated Ki-67 assessment method appears to be comparable to the visual method in synovial sarcoma and had a significant association to overall survival.",

author = "Laurila, {Riikka E} and B{\"o}hling, {Tom O} and Blomqvist, {Carl P} and Christina Karlsson and Tukiainen, {Erkki J} and Jussi Repo and Sampo, {Mika M}",

year = "2022",

doi = "10.21873/cdp.10070",

language = "English",

volume = "2",

pages = "7--14",

number = "1",

}

TY - JOUR

T1 - Visual Counting and Automated Image-analytic Assessment of Ki-67 and their Prognostic Value in Synovial Sarcoma

AU - Laurila, Riikka E

AU - Böhling, Tom O

AU - Blomqvist, Carl P

AU - Karlsson, Christina

AU - Tukiainen, Erkki J

AU - Repo, Jussi

AU - Sampo, Mika M

PY - 2022

Y1 - 2022

N2 - BACKGROUND: Ki-67 is a widely used proliferation marker reflecting prognosis in various tumors. However, visual assessment and scoring of Ki-67 suffers from marked inter-observer and intra-observer variability. We aimed to assess the concordance of manual counting and automated image-analytic scoring methods for Ki-67 in synovial sarcoma.PATIENTS AND METHODS: Tissue microarrays from 34 patients with synovial sarcoma were immunostained for Ki-67 and scored both visually and with 3DHistech QuantCenter.RESULTS: The automated assessment of Ki-67 expression was in good agreement with the visually counted Ki-67 (r Pearson =0.96, p<0.001). In a Cox regression model automated [hazard ratio (HR)=1.047, p=0.024], but not visual (HR=1.063, p=0.053) assessment method associated high Ki-67 scores with worse overall survival.CONCLUSION: The automated Ki-67 assessment method appears to be comparable to the visual method in synovial sarcoma and had a significant association to overall survival.

AB - BACKGROUND: Ki-67 is a widely used proliferation marker reflecting prognosis in various tumors. However, visual assessment and scoring of Ki-67 suffers from marked inter-observer and intra-observer variability. We aimed to assess the concordance of manual counting and automated image-analytic scoring methods for Ki-67 in synovial sarcoma.PATIENTS AND METHODS: Tissue microarrays from 34 patients with synovial sarcoma were immunostained for Ki-67 and scored both visually and with 3DHistech QuantCenter.RESULTS: The automated assessment of Ki-67 expression was in good agreement with the visually counted Ki-67 (r Pearson =0.96, p<0.001). In a Cox regression model automated [hazard ratio (HR)=1.047, p=0.024], but not visual (HR=1.063, p=0.053) assessment method associated high Ki-67 scores with worse overall survival.CONCLUSION: The automated Ki-67 assessment method appears to be comparable to the visual method in synovial sarcoma and had a significant association to overall survival.

U2 - 10.21873/cdp.10070

DO - 10.21873/cdp.10070

M3 - Article

C2 - 35400010

SN - 2732-7787

VL - 2

SP - 7

EP - 14

JO - Cancer diagnosis & prognosis

JF - Cancer diagnosis & prognosis

IS - 1

ER -

Visual Counting and Automated Image-analytic Assessment of Ki-67 and their Prognostic Value in Synovial Sarcoma

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